Megatron (adpacempertyrannidem): male-identified, incredibly pansexual, usually dominant (but not always), likes wild things like seekers, beastformers and Loki best.

Laserbeak (alittlebirbtoldme): female-identified, NOPE, what is sex can I eat it? Laserbeak is aroace and just wants to steal your shinies, spy on you for Soundwave, and eat your food if it won't make her sick.

Clobber (clobberintime): female-identifed, very uncertain about what she wants, really is looking for someone who wants her. Might be grey-ace because yes she's weird-looking but so are a lot of other Decepticons. I think tbh she is really young and inexperienced with her own feelings/emotions.

Ravage (don'tpetmeibite): genderfluid. Always male or female, so not really NB, but holy crap does she switch on a dime. He does have an issue with gender-neutral pronouns which is a cultural thing, but she'll tell you what to call her if you ask. Pan only with other robots. Poly by nature but monogamous with Soundwave by agreement.

Esmeral (esmeral): female-identified, heterosexual, occasionally very frustrated by being the lone hetero in a homonormative society. Engaged to a bi/poly fiance, she has a hall pass but feels weird about actually using it.

Jonathan Fairlight Gracen (fairlight): An actual human! Male-identified, cis but not really gender-conforming sometimes. Extremely gay. Also nervous about being thought transphobic because, while he isn't, he is like, extremely into dick. OC, married to Jim Profit in my main continuity.

Glit (glitterdoc): Male identified, cougaraider (the same type of robot that Ravage is), only attracted to humans (primarily due to trauma), doesn't care about gender.

Lotor (princeprocyon): is a ~7 year old child and doesn't care or really even know about any of this stuff.

Jim Profit (profit): cis male, human, in games/memes when not married to Fairlight, will do just about anything with anyone if it suits his self-interest or fits into his plans, pansexual, probably would have been panromantic if not badly abused by a woman

Rumble & Frenzy (redtwinbluetwin): I haven't played them long enough to be sure, but I know for a fact that Rumble at least is fully male-identified and they both like to double-team people about their size regardless of species.

Rosanna (rosie-the-riveting): female-identified, lesbian, same model chassis as Rumble & Frenzy (with mods) and likes girls around her size regardless of species.

Soundwave (soundwave-ascendant): male-identified, strictly interested only in other TFs on a physical level (so much squick with organics, they are squishy and feel weird) although he is a telepath/empath and primarily attracted to people's sparks/fields/minds. He is also monogamous with Ravage.

Stanford Pines (stanford-pines): sapio-pan-xeno-demisexual cis male human, does not fall in love with anyone who can't keep up with him mentally, is xeno enough to have more than once banged a triangle (now regrets this intensely), played for years in a game where he was married to a felinoid woman, Valiska, and a human from another dimension, Severin.

Pacifica (unpacified): Young adult nonbinary lesbian human. Her primary relationship is with a pansexual human woman, Mabel, but they have spent a lot of time around trolls and consider themselves to be (not actually very hateful) kismeses. Does quadrants. Is a bit of an ashen bicycle.

Valiska Courinna (valiska): cis-female Talassani (feline humanoid race), married to Ford Pines and Severin, grey ace with actually a whole lot of libido but it's only ever had two targets and they were both human males. She married and kept them both.

Buzzsaw (yellowblurred): male-identified. Unsure of his sexuality (or I am unsure of it anyway) but it's probably all tied up in that serial-killing problem he has. He gets off on making "art" out of people he's killed and is basically a bird-shaped robot Hannibal villain.

I have way more characters than this, these are the only ones on DW rn.